Titel: Genome, transcriptome and miRNome analysis of single cells

Termin: 27.8.2006, 17:00 Uhr, bis 1.9.2006, 20:00 Uhr

Veranstaltungsort:
Miltenyi Biotec GmbH
MACSmolecular Business Unit
Nattermannalle 1
50829 Köln

Referenten: Andreas Bosio, PhD (Köln, Germany) *** Bernhard Gerstmayer, PhD (Köln, Germany) *** C.A. Klein, PhD (München, Germany) *** Christoph Göttlinger, PhD (Köln, Germany) *** Harold Cremer, PhD (Marseille, France) *** James Eberwine, PhD (Philadelphia, USA) *** Jürgen Schmitz, PhD (Bergisch Gladbach, Germany) *** Kay Hofmann, PhD (Köln, Germany) *** Lukas Baran (Bernried, Germany) *** Mario Assenmacher, PhD (Bergisch Gladbach, Germany) *** Michael Barrett, PhD (Palo Alto, USA) *** Michael Brownstein, PhD (Rockville, USA) *** Prof. Andreas Radbruch (Berlin, Germany) *** Prof. Ralf Küppers (Essen, Germany) *** Rainer Gangnus, PhD (Bernried, Germany) *** Sebastian Pfeffer, PhD (Strasbourg, France) *** Silvia Rüberg, PhD (Köln, Germany) *** Stefan Joos, PhD (Heidelberg, Germany) *** Stephen D. Ginsberg, PhD (New York, USA) *** Victor Appay, PhD (Paris, France)

Weitere Informationen:
http://cwp.embo.org/w06-37/index.html

Kurzbeschreibung: This course is targeted at young postdoctoral researchers and Ph.D. students, who are interested in microarray based analysis of genomic DNA, mRNA and microRNA of single or few cells. The lectures cover mainly three parts:

i) isolation of single or few cells with a focus on microdissection, fluorescent activated cell sorting (FACS) and magnetic cell sorting (MACS)

ii) isolation and amplification of the RNA and DNA from single or few cells

iii) generation and interpretation of gene expression profiles using microarrays and bioinformatics.

Beside the methodological aspects, selected examples of single cell analysis in the field of e.g. neurobiology and immunology will be presented and discussed. Participants will be taught in groups (two participants per group) hands-on how to get and interpret transcriptome data from single or few cells when starting with complex cell mixtures or tissues. If participants wish to bring their own samples (e.g. biopsies, cell cultures, slices, purified RNA) they are asked to specify this on the application form. Course participants will be expected to present their current research work in form of posters and short oral presentations.

Kontakt: Herr Dr. Andreas Bosio
Miltenyi Biotec GmbH
MACSmolecular
Business Unit
Stoeckheimer Weg 1
50829 Köln
EMBO2006@miltenyibiotec.de.